Cloning, Expression and in silico Characterization of a Truncated Antiviral Protein Gene from Bougainvillea spectabilis Willd.
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چکیده
منابع مشابه
Synthesis and Cloning of cDNA Encoding an Antiviral Protein from the Leaves of Bougainvillea spectabilis Willd. (Nyctaginaceae)
Total RNA was extracted from the mature leaves of Bougainvillea spectabilis Willd. and messenger RNA (mRNA) was separated out. Using mRNA as template, complementary DNA (cDNA) was synthesized and amplified by Reverse Transcription coupled PCR using gene specific primer. A product of 750 base pair plus was selected based on the size of Bougainvillea antiviral protein (BAP). After elution the pro...
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The present study reports that the flower of Bougainvillea spectabilis Willd. has the potentiality to reduce sodium selenate and synthesize selenium nanoparticles. The exposure of aqueous sodium selenate to the flower broth of B.spectabilis leads to the synthesis of stable selenium nanoparticles within five days. The colour change of the reaction medium from purple to brown colour indicates the...
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Introduction: Omp31 is animmunodominant and protective antigen conserved in Brucella species and a good candidate for vaccine design. Material & methods: The present study aimed at in silico design of the truncated Omp31 (TOmp31) using bioinformatic tools and to express the selected form in Escherichia coli (E. coli) Results and conclusion: Various bioinformatically calculated scores for the ...
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گل کاغذی (Bougainvillea spectabilis Willd.) عضوی از تیره نیکتاژیناسه (Nyctaginaceae Juss.)، دارای اهمیتی اساسی در باغبانی و نیز علوم داروسازی، کشاورزی و محیطی است. علی‌رغم آن، نمو گل در این گونه هنوز به خوبی شناسایی نشده است. گل‌های دو جنسی و فاقد گلبرگ گل کاغذی، دارای یک گلپوش لوله‌ای پنج بخشی، 8 پرچم با میله‌های نابرابر و یک برچه است که توسط براکته‌های نمایان و قرمزرنگ احاطه می‌شود. اپی‌فیلی،...
متن کاملPurification and characterization of antiviral protein from silkworm fecal matter
Antiviral proteins (AVP), present in silkworm fecal matter, show activity against nuclear polyhedrosis virus (NPV) in vitro and in vivo. The extract of silkworm fecal matter prepared in phosphate buffer solution of pH 7.5 was subjected to 50% solid ammonium sulfate precipitation to enrich AVP, then which was dialyzed. The dialysate was applied to the column containing silica gel-G, the column e...
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ژورنال
عنوان ژورنال: International Journal of Current Microbiology and Applied Sciences
سال: 2019
ISSN: 2319-7692,2319-7706
DOI: 10.20546/ijcmas.2019.806.341